NIBSC - Shef-8

Cell Line Name	SHEF-8
Cell Line Status	Currently available
Cell Type	Human Embryonic
Grade	Research
Source	Human embryo / blastocyst
Source type	Frozen
Consent Status	Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.
Date cell line derived	26 January 2007
Ethical Statement	In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor	Pfizer
Depositing Centre	Pfizer
Country of Origin	United Kingdom
MUL Contact Details	Timothy Allsopp [Timothy.Allsopp@pfizer.com]
Journal Reference	Publications available
SCSC No.	SCSC-07-31
UKSCB Accession No.	R-08-015
Distribution Bank No.	D01
Passage No.	p17
Feeder Cell Type	Mouse embryo fibroblast
Basel Medium	KO-DMEM
Protein Source	KO-SR
Growth Factors	bFGF
Incubation Conditions	37°C, 5% CO2, >95% RH, Subculture: Enzyme subculture
Culture Morphology	Embryonic stem cell colonial morphology
Preservation Method	freezing (10% DMSO)
Viability	>75%
DNA Profile	Human
Karyotype	46,XX
Characterisation Panel	Positive for pluripotency markers
Sterility Test	No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen	Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen	Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR