NIBSC - NOTT-2

Cell Line Name	NOTT-2
Cell Line Status	Currently available
Cell Type	Human Embryonic
Grade	Research
Source	Human embryo / blastocyst
Source type	Fresh
Consent Status	Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.
Date cell line derived	02 May 2005
Ethical Statement	In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor	University of Nottingham
Depositing Centre	University of Nottingham
Country of Origin	United Kingdom
Depositor Contact Details	BB-Contract-Request@nottingham.ac.uk
Journal Reference	Publications available
SCSC No.	SCSC-06-17
UKSCB Accession No.	R-07-002
Distribution Bank No.	D01
Passage No.	p19
Feeder Cell Type	Mouse embryo fibroblast
Basel Medium	KO-DMEM
Protein Source	FBS
Growth Factors	bFGF and LIF
Incubation Conditions	37°C, 5% CO2, >95% RH, Subculture: manual cutting
Culture Morphology	Embryonic stem cell colonial morphology
Preservation Method	Vitrified
Viability	>80%
DNA Profile	Human
Karyotype	46,XY
Characterisation Panel	Confirmed SSEA-1 Low; Confirmed Positive for SSEA-3/SSEA-4, TRA-1-60, TRA-1-81, TRA-1-85, TRA-2-54, OCT3/4; Awaiting Confirmation SOX 2
Sterility Test	No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen	Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen	Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR