NIBSC - NCL-4

Cell Line Name	NCL-4
Cell Line Status	Currently available
Cell Type	Human Embryonic
Grade	Research
Source	Human embryo / blastocyst
Source type	Fresh
Consent Status	Donor consent was reviewed by the UK Steering Committee and was found to be consistent with Human Fertilisation and Embryology Authority (HFEA) guidelines for centres deriving human embryonic stem cell lines under HFEA license and the requirements of UK le
Date cell line derived	30 October 2004
Ethical Statement	In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor	Newcastle Centre for Life
Depositing Centre	Newcastle Fertility Centre at LIFE
Country of Origin	United Kingdom
MUL Contact Details	legal@nesci.ac.uk
Journal Reference	Derivation of hes cells from developing and arrested embryos. Zhang X, StojkovicM et al. Stem Cells 2006 Dec; 24(12) 2669-2676
SCSC No.	SCSC-06-46(3)
UKSCB Accession No.	R-07-016
Distribution Bank No.	D01
Passage No.	p28
Feeder Cell Type	Mouse embryo fibroblast
Basel Medium	KO-DMEM
Protein Source	KO-SR
Growth Factors	bFGF
Incubation Conditions	37°C, 5% CO2 , Subculture: enzymic
Culture Morphology	Embryonic stem cell colonial morphology
Preservation Method	Frozen / DMSO
Viability	>80%
DNA Profile	Human
Karyotype	46,XY
Characterisation Panel	Awaiting Confirmation of Characterisation Panel Tests
Sterility Test	No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen	Mycoplasma not detected by Mycoplasma PCR or Culture