NIBSC - NCL-2

Cell Line Name	NCL-2
Cell Line Status	Currently available
Cell Type	Human Embryonic
Grade	Research
Source	Human embryo / blastocyst
Source type	Fresh
Consent Status	Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.
Date cell line derived	14 March 2005
Ethical Statement	In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor	Newcastle Fertility Centre at LIFE
Depositing Centre	Newcastle Fertility Centre at LIFE
Country of Origin	United Kingdom
MUL Contact Details	legal@nesci.ac.uk
Journal Reference	Publications available
SCSC No.	SCSC-06-46(1)
UKSCB Accession No.	R-07-14
Distribution Bank No.	DO1
Passage No.	p27
Feeder Cell Type	Human
Basel Medium	KO-DMEM
Protein Source	KO-SR
Growth Factors	bFGF
Incubation Conditions	37°C, 5% CO2 , Subculture: enzymic
Culture Morphology	Embryonic stem cell colonial morphology
Preservation Method	Frozen / DMSO
Viability	50-80%
DNA Profile	Human
Karyotype	46,XX
Characterisation Panel	Awaiting Confirmation of Characterisation Panel Tests
Sterility Test	No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen	Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen	Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR