NIBSC - Edi-4

Cell Line Name	Edi-4
Cell Line Status	Currently available
Cell Type	Human Embryonic
Grade	Research
Source	Human embryo / blastocyst
Source type	Cryopreserved
Consent Status	Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.
Date cell line derived	24 July 2006
Ethical Statement	In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor	University of Edinburgh
Depositing Centre	University of Edinburgh
Country of Origin	United Kingdom
MUL Contact Details	RSO.Contracts@ed.ac.uk
Journal Reference
SCSC No.	SCSC-07-13
UKSCB Accession No.	R-07-025
Distribution Bank No.	d01
Passage No.	p17
Feeder Cell Type	Human embryo fibroblast
Basel Medium	DMEM /F-12
Protein Source	BSA
Growth Factors	bFGF & LIF
Incubation Conditions	37°C, 5% CO2, >95% RH, manual dissection
Culture Morphology	Embryonic stem cell colonial morphology
Preservation Method	Vitrification
Viability	Viable
DNA Profile	Human
Karyotype	Awaiting result
Characterisation Panel	Awaiting Confirmation of Characterisation Panel Tests
Sterility Test	No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen	Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen	Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR