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The National Institute for Biological Standards and Control

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BJNhem19

Cell Line Name BJNhem19
Cell Line Status Currently available
Cell Type Human Embryonic
Grade Research.
Source Human embryo / blastocyst
Source type Fresh
Consent Status Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.  
Date cell line derived 02 April 2007
Ethical Statement In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor JNCASR, Bangalore
Depositing Centre JNCASR, Bangalore
Country of Origin India
MUL Contact Details  inamdar@jncasr.ac.in
Journal Reference

 Publications available

SCSC No. SCSC-07-43(1)
UKSCB Accession No. R-08-022
Distribution Bank No. d01  
Passage No. p+9
Feeder Cell Type Mouse embryo fibroblast
Basel Medium KO-DMEM
Protein Source KO-SR
Growth Factors bFGF & LIF
Incubation Conditions 37°C, 5% CO2, >95% RH, Subculture: Enzyme subculture  
Culture Morphology Embryonic stem cell colonial morphology
Preservation Method freezing (10% DMSO)  
Viability >80%
DNA Profile Human
Karyotype 46, XY
Characterisation Panel Positive for pluripotency markers
Sterility Test No contamination detected by EU Pharmacopeia Sterility Test
Mycoplasm Screen Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR
 
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