Dr Sandrine Vessillier

Dr Sandrine Vessillier joined NIBSC in 2012. She has over 15-years’ experience in development of biotherapeutics (molecular engineering, production, purification and characterisation) and in preclinical testing using models of acute and chronic inflammation.

She performs research in cellular immunology to determine the mechanisms of pro-inflammatory mediator release from immune cells following exposure to biological medicines, eg. immunotherapies. She develops novel assays for predicting adverse responses to biotherapeutics.

She is also interested in the study of inflammation in arthritis disease to identify pathways/therapeutics that slow down inflammation and facilitate tissue repair without compromising the immune defence against infection. Her main interests include cytokines, monoclonal antibodies and matrix degrading enzymes.

She supervises the development of reference reagents for flow cytometry applications such as lyophilised cell standards for process controls for regenerative medicine, cell based diagnostics and immune monitoring of treatment efficacy.

Publications

Vessillier, S., M. Fort, L. O’Donnell, H. Hinton, K. Nadwodny, J. Piccotti, P. Rigsby, K. Staflin, R. Stebbings and D. Mekala, Willingham, A. and B. Wolf (2020). "Development of the first reference antibody panel for qualification and validation of cytokine release assay platforms-Report of an international collaborative study." Cytokine: X: 100042. https://doi.org/10.1016/j.cytox.2020.100042

 
Deepa Rajagopal, Linhua Tian, Shiqiu Xiong, Lili Wang, Jonathan Campbell, Luisa Saraiva, Sandrine Vessillier An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes, Journal of Immunology and Regenerative Medicine 2020 https://doi.org/10.1016/j.regen.2020.100032

Faruqui N, Kummrow A, Fu B, Divieto C, Rojas F, Kisulu F, Cavalcante JJV, Wang J, Campbell J, Martins JL, Choi JH, Sassi MP, Zucco M, Vonsky M, Vessillier S, Zou S, Fujii SI, Ryadnov MG. Front Cellular Metrology: Scoping for a Value Proposition in Extra- and Intracellular Measurements. Bioeng Biotechnol. 2020 Jan 14;7:456. doi: 10.3389/fbioe.2019.00456. eCollection 2019. Review

Eyles, J. E., Vessillier, S.*, Jones, A., Stacey, G., Schneider, C. K., Price, J. (2018) Cell TherapyProducts: Focus on Issues with Manufacturing and Quality Control of Chimeric Antigen Receptor T‐cell therapies J. Chem. Technol. Biotechnol. https://doi.org/10.1002/jctb.5829

Saraiva, L., Wang, L., Kammel, M., Kummrow, A., Atkinson, E., Lee, J.Y., Yalcinkaya, B., Akgöz, M., Höckner, J., Ruf, A., Engel, A., Zhang, Yu-Zhong., O’Shea, O., Sassi, M. P., Divieto, C., Lekishvili, T., Campbell, J.J., Liu, Y., Wang, J., Stebbings, R., Gaigalas, A.K., Rigsby P., Neukammer, J. and Vessillier S. (2019) Comparison of volumetric and bead-based counting of CD34 cells by singleplatform flow cytometry, Cytometry part B: Clinical cytometry https://doi.org/10.1002/cyto.b.21773

Whitfield, S. J. C. , Taylor, C.,  Risdall, J. E., Griffiths, G. D. , Jones, J. T. A., Williamson, E. D.,  Rijpkema, S., Saraiva, L. , Vessillier, S.,  Green , A. C. and Carter, A. J. (2017) Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology. The Journal of  Immunology, 198 (10): 3989-3998

Tilwani, R.K., Vessillier, S., Pingguan-Murphy, B., Lee, D.A., Bader, D.L. and Chowdhury, T.T. (2016) Oxygen tension modulates the effects of TNFα in compressed chondrocytes. Inflammation Research, 66 (1): 49-58

Peake, N., Bader, DL, Vessillier, S., Ramachandran, M., Salter, D.M., Hobbs, AJ. and Chowdhury, TT. (2015) C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes. Biochemical and Biophysical Research Communications, 465 (4): 784-789

Vessillier, S.,   Eastwood, D., Fox, B., Swaminathan, S., Sathish, J. Thorpe, S.J., Thorpe, R. and Stebbings, R. (2015) Cytokine release assays for the prediction of therapeutic mAb safety in first-in man trials - Whole blood cytokine release assays are poorly predictive for TGN1412 cytokine storm. Journal of immunological methods424:43-52

Reed, D.M., Paschalaki, K.E., Starke, R.D., Mohamed, N.A, Sharp, G., Fox, B., Eastwood, E., Bristow, A., Ball, C., Vessillier, S.,  Hansel, T.T., Thorpe, S.J., Randi, A.M.,  Stebbings, R. and Mitchell, J.A. (2015) An autologous endothelial cell: PBMC assay that detects cytokine storm responses to biologics. FASEB Journal, 29(6): 2595-602.

Mullen, L., Adams, G., Vessillier, S., Rigby, A., Sclanders, M., Mittal, G., Colston, J., Fatah, R., Subang, C., Francis-West, P., Koster, M., Hauser, H., Layward, L., Annenkov, A., Al-Izki, S., Pryce, G., Bolton, C., Baker, D., Gould, D. J. and Chernajovsky, Y. (2014) Latent cytokines for targeted therapy of inflammatory disorders. Expert Opin Drug Deliv, 11(1): 101-110

Mullen, L., Rigby, A., Sclanders, M., Adams, G., Mittal, G., Colston, J., Fatah, R., Subang, C., Foster, J., Francis-West, P., Koster, M., Hauser, H., Layward, L., Vessillier, S., Annenkov, A., Al-Izki, S., Pryce, G., Bolton, C., Baker, D., Gould, D. J. and Chernajovsky, Y. (2014)  Latency can be conferred to a variety of cytokines by fusion with latency-associated peptide from TGF-β. Expert Opin Drug Deliv. 11 (1): 5-16

Koutsokeras, A., Purkayashta, N., Rigby,A., Subang, M.C., Sclanders, M., Vessillier, S., Mullen, L., Chernajovsky,Y., Gould,D (2014) Generation of an efficiently secreted cell, penetrating NF-κB inhibitor. FASEB J, 28(1): 373-381

Parker,E., Vessillier,S., Pingguan-Murphy,B., Wan Abas,W.A.B., Bader,D.L, and Chowdhury,T.T (2013) Low oxygen tension increased fibronectin fragment induced catabolic activities – response prevented with biomechanical signals. Arthritis Research & Therapy, 15 (5): R163, doi:10.1186/ar4346

Man,Y.K.S., DiCara,D., Chan, N., Vessillier,S., Mather,S.J., Rowe, M.L., Howard, M. J., Marshall, J.F. & Nissim, A. (2013) Structural Guided Scaffold Phage Display Libraries as a Source of Bio-Therapeutics. Plos one,8 (8), e70452. doi: 10.1371/journal.pone.0070452

Mullen, L., Adams,G., Foster, J., Vessillier,S., Koster,M., Hauser,H., Layward,L., Gould,D. and  Chernajovsky,Y. (2013). A comparative study of matrix metalloproteinase and aggrecanase mediated release of latent cytokines at arthritic joints. Ann Rheum Dis doi:10.1136/annrheumdis-2013-203513

Mullen, L., Adams, G., Fatah, R., Gould, D., Rigby, A., Sclanders, M., Koutsokeras, A., Mittal, G., Vessillier, S. & Chernajovsky, Y. (2013) Development of latent cytokine fusion proteins. In Fusion protein technologies for biopharmaceuticals: Applications and Challenges (S.R. Schmidt), Wiley-Blackwell

Vessillier, S., Adams, G., Montero-Melendez, T., Jones, R., Seed, M., Perretti, M. & Chernajovsky, Y. (2012) Molecular engineering of short-half-life small peptides (VIP, αMSH and γ3MSH ) fused to latent associated peptide (LAP) results in improved anti-inflammatory therapeutics. Ann Rheum Dis, 71 (1), 143-9

Duchene, J., Cayla, C., Vessillier, S., Scotland, R., Yamashiro, K., Lecomte, F., Syed, I., Vo, P., Marrelli, A., Pitzalis, C., Cipollone, F., Schanstra, J., Bascands, JL., Hobbs, AJ., Perretti, M. & Ahluwalia, A. (2009) Laminar shear stress regulates endothelial kinin B1 receptor expression and function. Potential implication in atherogenesis. Arterioscler Thromb Vasc Biol., 29 (11), 1757-63

Vessillier, S., Adams, G. & Chernajovsky, Y. (2004) Latent cytokines: development of novel cleavage sites and kinetic analysis of their differential sensitivity to MMP-1 and MMP-3. Protein Eng Des Sel., 17 (12), 829-835

Nissim, A., Gofur, Y., Vessillier, S., Adams, G. & Chernajovsky, Y. (2004) Methods for targeting biologicals to specific disease sites. Trends Mol Med., 10 (6), 269-274.

Chernajovsky, Y., Vessillier, S. & Adams, G (2004) Targeting therapeutic molecules to sites of disease. Discov. Med., 4 (20), 30-32

Chernajovsky, Y., Vessillier, S., Adams, G., Gofur, Y., Subang, M. C., Annenkov, A., Favorov, P., Daly, G. & Gould, D. (2003) Development of new molecules, vectors and cells for therapy of arthritis. Joint Bone Spine, 70 (6), 474-476

Chernajovsky, Y., Vessillier, S., Adams, G., Gofur, Y., Subang, M. C., Annenkov, A., Favorov, P., Daly, G. & Gould, D. (2003) Développement de nouvelles molécules, vecteurs et cellules thérapeutiques dans les arthrites. Revue du rhumatisme, 70 (10), 846-848

Adams, G., *Vessillier, S., Dreja, H. & Chernajovsky, Y. (2003) Targeting cytokines to sites of inflammation. Nat. Biotechnol., 21(11), 1314-1320

Franceschini, N., Segatore, B., Perilli, M.G., Vessillier, S., Franchino, L & Amicosante, G. (2002) Meropenem stability to β-lactamase hydrolysis and comparative "in vitro" activity against several β-lactamase-producing gram-negative strains. J. Antimicrob. Chemother., 49 (2), 395-398.

Vessillier, S., Docquier, J.D., Rival, S., Galleni, M., Amicosante, G., Rossolini, G.M. & Franceschini, N. (2002) Overproduction and biochemical characterization of the Chryseobacterium meningosepticum BlaB metallo-β-lactamase. Antimicrob. Agents Chemother., 46 (6), 1921-1927

Mollard, C., Moali, C., Papamicael, C., Damblon, C., Vessillier, S., Amicosante, G., Schofield, C. J., Galleni, M., Frere, J.M. & Roberts, G.C.K. (2001) Thiomandelic acid, a broad-spectrum inhibitor of zinc β-lactamases: kinetic and spectroscopic studies. J. Biol. Chem., 276 (48), 45015-45023

Vessillier, S., Delolme, F., Bernillon, J., Saulnier, J. & Wallach, J.M. (2001) Hydrolysis of glycine-containing elastin pentapeptides by LasA, a metalloelastase from Pseudomonas aeruginosa. Eur. J. Biochem., 268 (4), 1049-1057

Vessillier, S., Bernillon, J., Saulnier, J. & Wallach, J. (1997) Capillary electrophoresis in the assay of the hydrolysis of glycine-containing peptides by a protease from Pseudomonas aeruginosa. J. Chromatogr. A, 776 (1), 133-137

Besson, C., Vessillier, S., Gonzales, T., Saulnier, J. & Wallach, J. (1994) Conductimetric assay of pyroglutamyl peptidase activity. Anal. Chim. Acta., 294, 305-309