Publications
Lockyer K, Gao F, Francis RJ, Eastwood D, Khatri B, Stebbings R, Derrick JP,
Bolgiano B. (2020) Higher mass meningococcal group C-tetanus toxoid vaccines conjugated with carbodiimide correlate with greater immunogenicity.
Vaccine 38, 2859-2869.
https://doi.org/10.1016/j.vaccine.2020.02.012
Hitri K., Kuttel MM, De Benedetto G, Lockyer K, Gao F, Hansal P, Rudd TR, Beamish E, Rijpkema S, Ravenscroft N, Bolgiano B. (2019) O-acetylation of typhoid capsular polysaccharide confers rigidity and immunodominance by masking additional epitopes. Vaccine 37, 3866-3875.https://doi.org/10.1016/j.vaccine.2019.05.050
Gao F, Swann C, Rigsby P, Rijpkema S, Lockyer K, Logan A, Bolgiano B & Vi IS Working Group. (2019) Evaluation of two WHO First International Standards for Vi PS from Citrobacter freundii and Salmonella enterica subspecies enterica serovar Typhi. Biologicals 57, 34-45. https://doi.org/10.1016/j.biologicals.2018.11.004
Beresford NJ, Martino A, Feavers IM, Corbel MJ, Bai X, Borrow R,
Bolgiano B. Quality, immunogenicity and stability of meningococcal serogroups ACWY-CRM
197, DT and TT glycoconjugate vaccines.
Vaccine 2017 Jun 10;35(28):3598-3606.
http://dx.doi.org/10.1016/j.vaccine.2017.03.066
Vipond C, Swann C, Dougall T, Rigsby P, Gao F, Bolgiano B and Working Group. Evaluation of candidate International Standards for meningococcal serogroups A and X polysaccharide. Biologicals, 2017 May 5;47:33-45. http://dx.doi.org/10.1016/j.biologicals.2017.03.001
Voysey M, Sadarangani M, Clutterbuck E, Bolgiano B, Pollard AJ. The impact of administration of conjugate vaccines containing cross reacting material on Haemophilus influenzae type b antibody responses in infants: A systematic review and meta-analysis of randomised controlled trials. Vaccine. 2016; 34: 3986-92. http://dx.doi.org/10.1016/j.vaccine.2016.06.038
Asokanathan C, Yuen C-T, Kmiec D, Wheeler JX, Markey K, Kelso I, Bolgiano B, He Q, Mooi FR, Xing D. (2015) Functional differences in pertussis toxins from Bordetella pertussis clinical isolates as determined by in vitro and in vivo assays. Clinical & Experimental Pathology 5, 243
Mawas F, Burkin K, Dougall T, Saydam M, Rigsby P, Bolgiano B, the Hib PRP IS Working Group. (2015) International collaborative study for establishment of the 2nd WHO International Standard for Haemophilus influenzae type b polysaccharide. Biologicals 43, 492-503. http://dx.doi.org/10.1016/j.biologicals.2015.07.006
Otto RBD, Burkin K, Mulla SE, Crane DT, Bolgiano B. (2015) Patterns of binding of aluminum-containing adjvuants to Haemophilus influenza type b and meningococcal group C conjugate vaccines and components. Biologicals 43, 355-362. https://dx.doi.org/10.1016/j.biologicals.2015.06.008
Lockyer K, Gao F, Derrick JP, Bolgiano B. (2015) Structural correlates of carrier protein recognition in tetanus toxoid conjugated bacterial protein vaccines. Vaccine 33, 1345-1352. http://dx.doi.org/10.1016/j.vaccine.2015.01.046
Gao F, Lockyer K, Burkin K, Crane DT, Bolgiano B. (2014) A physico-chemical assessment of the thermal stability of pneumococcal conjugate vaccine components. Human Vaccines & Immunotherapeutics 10, 2744-2753. http://dx.doi.org/10.4161/hv.29696
Xing D, Asokanathan C, Xu YH, Bolgiano B, Douglas-Bardsley, Zhang S, Wang J, Corbel M. (2014) Relationship of immunogenicity to protective potency in acellular pertussis vaccines. Human Vaccines & Immunotherapeutics 10, 2066-2073. https://doi.org/10.4161/hv.28765
Bristow AF, Bird C, Bolgiano B, Thorpe R. (2012) Regulatory requirements for therapeutic proteins: the relationship between the conformation and biological activity of filgrastim. Pharmeuropa Bio & Scientific Notes, October 2012, 103-117.
Xie O, Bolgiano B, Gao F, Lockyer F, Swann, C, Jones C, Selrieu, Njanpop-Lafourcade B-M, Tamekloe TA, Pollard AJ, Norheim G. (2012) Characterization of size, structure and purity of a serogroup X Neisseria meningitidis polysaccharide, and development of an assay for quantification of human antibodies. Vaccine 30, 5812-5823. http://dx.doi.org/10.1016/j.vaccine.2012.07.032
Vipond C, Mulloy B, Rigsby P, Burkin K, Bolgiano B and the MenC IS Working Group. (2012) Evaluation of a candidate International Standard for Meningococcal Group C polysaccharide. Biologicals 40, 353-363. 10.1016/j.biologicals.2012.03.003
Martino A, Magagnoli C, De Conciliis G, D;Ascenzi S, Forster MJ, Allen L, Brookes C, Taylor S, Bai X, Findlow J, Feavers IM, Rodger A, Bolgiano B. (2012) Structural characterization, stability and antibody recognition of chimeric NHBA-GNA1030: An investigational vaccine component against Neisseria meningitidis. Vaccine 30, 1330-1342. https://doi.org/10.1016/j.vaccine.2011.12.066
Xu Y, Zhang S, Bolgiano B, Tan Y, Asokanathan C, Zhang H, Xing D, Wang J. (2011) Comparison of recombinant and native pertactin of Bordetella pertussis. Vaccine, 24, 1974-1980. http://dx.doi.org/10.1016/j.vaccine.2010.12.110
Saydam M, Burkin K, Care R, Rigsby P, Bolgiano B, Mawas F. (2010) Immunogenicity and thermal stability of a combined vaccine against Haemophilus influenzae type b and Neisseria meningitides serogroup C diseases. Vaccine, 28, 6228-6234. http://dx.doi.org/10.1016/j.vaccine.2010.07.005
Otto RBD, Crane DT & Bolgiano B. (2007) A study of physico-chemical interactions between Haemophilus influenzae type b and meningococcus group C conjugate vaccines. African Health Sciences, 7, 190-196 .PMID: 21499483
Bolgiano B, Mawas F, Burkin K, Crane DT, Saydam M, Rigsby P & Corbel MJ. (2007) A retrospective study on the quality of Haemophilus influenzae type b vaccines used in the U.K. between 1996 and 2004. Human Vaccines, 3, 176-182. DOI: 10.4161/hv.3.5.4352
Mawas F, Bolgiano B, Rigsby P, Crane D, Belgrave D & Corbel MJ. (2007) Evaluation of the saccharide content and stability of the first WHO International Standard for Haemophilus influenzae b capsular polysaccharide. Biologicals, 35,235-245. http://dx.doi.org/10.1016/j.biologicals.2007.01.003
Ho MM, Bolgiano B & Corbel MJ. (2007) Response to letter. Don’t shoot the [Carrier]. Human Vaccines 3, 41. DOI: 10.4161/hv.3.2.3875
Qazi O, Bolgiano B, Crane D, Svergun DI, Konarev PV, Yao Z-P, Robinson CV, Brown KA & Fairweather N. (2007) The Hc fragment of tetanus toxin forms stable, concentration-dependent dimers via an intermolecular disuphide bond. J Mol Biol 365, 123-134. https://doi.org/10.1016/j.jmb.2006.09.050
Qazi O, Sesardic D, Tierney R, Soderback Z, Crane D, Bolgiano B & Fairweather N. (2006) Reduction of ganglioside binding activity of tetanus toxin Hc fragment destroys immunogenicity: implications for development of novel tetanus vaccines. Infection and Immunity 74, 4884-4891. doi: 10.1128/IAI.00500-06
Ho MM, Bolgiano B, Martino A, Kairo SK, Corbel MJ. (2006) Preclinical laboratory evaluation of a bivalent Staphylococcus aureus saccharide exotoxin A protein conjugate vaccine. Human Vaccines, 2, 89-98. DOI: 10.4161/hv.2755
Suker J, Feavers IM, Corbel MJ, Jones C & Bolgiano B (2004) Control and ot release of meningococcal group C conjugate vaccines. Expert Rev. Vaccines 3, 533-540. https://doi.org/10.1586/14760584.3.5.533
Jeffs SA, Goriup S, Kebble B, Crane D, Bolgiano B, Sattentau Q, Jones S & Holmes H. (2004) Expression and characterisation of recombinant oligomeric envelope glycoproteins derived from primary isolates of HIV-1. Vaccine 22, 1032-1046. https://doi.org/10.1016/j.vaccine.2003.08.042
Fowler S, Xing D K-L, Bolgiano B, Yuen CT, Corbel, MJ. (2003) Modification of the catalytic and binding subunits of pertussis toxin by formaldehyde: effects on toxicity and immunogenicity. Vaccine 21, 2329-2337.https://doi.org/10.1016/S0264-410X(03)00104-X
Fowler S, Byron O, Jumel K, Xing D, Corbel MJ, Bolgiano B. (2003) Novel configurations of high molecular weight species of pertussis toxin vaccine component. Vaccine 21, 2678-2688. https://doi.org/10.1016/S0264-410X(03)00105-1
Ho MM, Mawas F, Bolgiano B, Lemercinier X, Crane D, Huskisson R, Corbel M. (2002) Physico-chemical and immunological examination of the thermal stability of tetanus toxoid conjugate vaccines. Vaccine 20, 3509-3522. https://doi.org/10.1016/S0264-410X(02)00342-0
Jumel K, Ho MM, Bolgiano B. (2002) Evaluation of Meningococcal C oligosaccharide conjugate vaccines by size exclusion chromatography/multi-angle laser light scattering. Biotechnology Applied Biochemistry 36, 219-226. https://doi.org/10.1042/BA20020066
Bolgiano B, Mawas F, Yost SE, Crane DT, Lemercinier X, Corbel MJ. (2001) Effect of physico-chemical modification on the immunogenicity of Haemophilus influenzae type b oligosaccharide-CRM197 conjugate vaccines. Vaccine, 19, 3189-3200. DOI: 10.1016/s0264-410x(01)00024-x
Ho MM, Lemercinier X, Bolgiano B, Crane DT, Corbel MJ. (2001) Conformational stability of the subunit components of meningococcal oligosaccharide C-CRM197 conjugate vaccines. Biotechnology Applied Biochemistry, 33, 91-98. https://doi.org/10.1042/BA20000078
Bolgiano B, Fowler S, Turner K, Sesardic D, Xing DK-L, Crane DT, Corbel MJ. (2000) Monitoring of protein vaccine components by circular dichroism, fluorescence spectroscopy and size-exclusion chromatography: diphtheria, pertussis and tetanus toxoids. Developments in Biologicals, 103, 51-59
Fowler S, Bolgiano B, Byron O, Xing D, Corbel MJ. (2000) Pertussis toxin: effects of formaldehyde on structural properties. Intl J Med Micro, 290, A63
Frech C, Hilbert AK, Hartmann G, Mayer K, Sauer T, Bolgiano B. (2000) Physicochemical analysis of purified diphtheria toxoids: Is toxoided then purified the same as purified then toxoided? Developments in Biologicals, 103, 205-215.
Ho MM, Bolgiano B, Corbel MJ. (2000) Assessment of the stability and immunogenicity of meningococcal oligosaccharide C-CRM197 conjugate vaccines. Vaccine, 19, 716-725. https://doi.org/10.1016/S0264-410X(00)00261-9
Ho MM, Lemercinier X, Bolgiano B, Corbel MJ. (2000) Monitoring stability of meningococcal group C conjugate vaccines; correlation of physico-chemical methods and immunogenicity assays. Developments in Biologicals, 103, 139-150.
Jepson M, Crane D, Basak A, Naylor C, Titball RW, Bolgiano B. (2000) Structural analysis and calcium binding of the C-domain of C. perfringens alpha-toxin. Intl J Med Micro, 290.
Sasiak AB, Bolgiano B, Crane DT, Hockley DJ, Corbel MJ, Sesardic D. (2000) Comparison of in vitro and in vivo methods to study stability of PLGA microencapsulated tetanus toxoid vaccines. Vaccine, 19, 694-705.https://doi.org/10.1016/S0264-410X(00)00266-8
Bolgiano B, Crane DT, Xing D, Williams L, Jones C, Corbel MJ. (1999)Physico-chemical analysis of Bordetella pertussis antigens. Biologicals 27,155-162. https://doi.org/10.1006/biol.1999.0201
Crane, DT, Bolgiano B, Jones C, Corbel MJ, Sasiak A, Sesardic D, Xing D. (1999) Physicochemical methods for testing consistency of production for toxoid and glyco-conjugate vaccines. Developments in Biological Standandardization. 101, 324.
Corbel MJ, Xing DK-L, Bolgiano B, Hockley DJ. (1999) Approaches to the control of acellular pertussis vaccines. Biologicals 27, 133-141.
Jeffs S, Jones S, Kebble B, Holmes J, Bolgiano B, Crane D, Stebbings R. (1999) Characterisation of oligomeric recombinant envelope glycoproteins from primary isolates of HIV-1. The Fourth European Conference on Experimental AIDS Research, pp. 195-200, Monduzzi Editore, Bologna.
Jepson M, Howells A, Bullifent HL, Bolgiano B, Crane D, Miller J, Holley J, Jayasekera P, Titball RW. (1999) Differences in the carboxy-terminal (putative phospholipid binding) domains of Clostridium perfringens and Clostridium bifermentans phospholipases C influence the hemolytic and lethal properties of these enzymes. Infection Immunity 67, 3297-3301. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC116509/
Lemercinier X, Crane D, Gee C, Austin, S, Bolgiano B, Jones C. (1999) New methods for the characterization of biopharmaceuticals: Conjugate vaccines against Haemophilus influenzae type b. Developments in Biological Standardization 101, 177-183. https://pubmed.ncbi.nlm.nih.gov/10566791/
Naylor CE, Jepson M, Crane DT, Titball RW, Miller J, Basak AK, Bolgiano B. (1999) Characterisation of the calcium-binding C-terminal domain of Clostridium perfringens alpha-toxin. J Molecular Biology 294, 757-770. DOI: 10.1006/jmbi.1999.3279
Tarelli E, Mire-Sluis A, Tivnann HA, Bolgiano B, Crane DT, Gee C, Lemercinier X, Athayde ML, Sutcliffe N, Corran PH, Rafferty B. (1998) Recombinant human albumin as a stabilizer for biological materials and for the preparation of international reference reagents. Biologicals 26, 331-346. https://doi.org/10.1006/biol.1998.0163
Crane DT, Bolgiano B, Jones C. (1997) Comparison of the diphtheria mutant toxin, CRM197, with a Haemophilus influenzae type-b polysaccharide-CRM197 conjugate by optical spectroscopy. European Journal of Biochemistry, 246, 320-327. https://doi.org/10.1111/j.1432-1033.1997.00320.x
Jones C, Crane DT, Lemercinier X, Bolgiano B, Yost, SE. (1996) Physicochemical studies of the structure and stability of polysaccharide-protein conjugate vaccines. Developments in Biological Standardization 87, 143-151. https://pubmed.ncbi.nlm.nih.gov/8854011/
Jones C, Bolgiano, B. (1996) Applications of circular dichroism and fluorescence spectroscopy to probe protein-protein interactions. Nanobiology, 4, 1-15.
Xing DK-L, Crane DT, Bolgiano B, Corbel MJ, Jones C, Sesardic D.(1996) Physicochemical and immunological studies on the stability of free and microsphere-encapsulated tetanus toxoid in vitro. Vaccine 14, 1205-1213. https://doi.org/10.1016/S0264-410X(96)00032-1
Crane DT, Bolgiano B, Jones C, Edgell TA, Pring JB, Gaffney, PJ. (1994) Stability of the glycoenzyme tissue plasminogen activator studied by circular dichroism. Biochemical Society Transactions 22, 105S. DOI: 10.1042/bst023105s
Bourne Y, Bolgiano B, Liao D-I, Strecker G, Cantau P, Herzberg O, Feizi T Cambillau C. (1994) X-ray crystallography reveals cross-linking of mammalian lectin (Galectin-1) by biantennary-type saccharides. Nature Structural Biology 1, 863-870.
Bourne Y, Bolgiano B, Nesa M-P, Penfold P, Johnson D, Feizi T, Cambillau C. (1994) Crystallisation and preliminary X-ray diffraction studies of the soluble 14kDa b-galactoside-binding lectin from bovine heart. Journal of Molecular Biology 235, 787-789.
Bolgiano B, Salmon I, Poole R. (1993) Reactions of the membrane-bound cytochrome bo terminal oxidase of Escherichia coli with carbon monoxide and oxygen. Biochim Biophys Acta 1141, 95-104.
Bolgiano B, Davies HC, Poole, RK. (1991) Using the bacterium Paracoccus denitrificans and other "runaway mitochondria" as classroom models for respiratory electron transport. Biochemical Society Transactions 19, 976-981.
Bolgiano B, Salmon I, Ingledew WJ, Poole, RK. (1991) Redox analysis of the cytochrome o-type oxidase of Escherichia coli reveals three redox components. Biochemical Journal 274, 723-730.
Salerno JC, Bolgiano B, Ingledew WJ. (1990) Heme-copper and heme-heme interactions in the cytochrome bo-containing quinol oxidase of Escherichia coli. J Biol Chem 265, 4364-4368.
Bolgiano B, Smith L, Davies, HC. (1989) Electron transport reactions in a cytochrome c-deficient mutant of Paracoccus denitrificans. Biochimica Biophysica Acta 973, 227-234.
Smith L, Bolgiano B, Davies HC (1988) Kinetics of the interactions of the cytochrome c oxidase of Paracoccus denitrificans with Paracoccus and mitochondrial cytochrome c. Prog. Clin. Biol. Res. 274, 619-635.
Smith L, Bolgiano B, Davies, HC. (1988) Kinetics of the interaction of cytochrome c oxidase of Paracoccus denitrificans with Paracoccus and mitochondrial cytochrome c. In King, T (ed.) "Oxidases and Related Redox Systems" pp. 619-635. AR Liss, New York.
Bolgiano B, Smith, L, Davies, HC. (1988) Kinetics of the interaction of the cytochrome c oxidase of Paracoccus denitrificans with its own and bovine cytochrome c. Biochimica Biophysica Acta 933, 341-350.
Contribution to non-authored technical publications
Recommendations to assume the quality, safety and efficacy of typhoid conjugate vaccines. Replacement of WHO Technical Report Series, No. 987, Annex 3. Geneva, World Health Organization; 2020. https://www.who.int/publications/m/item/tcv71-recommendations
Meeting Report - WHO Informal consultation on amendments to WHO Guidelines on the quality, safety and efficacy of typhoid conjugate vaccines. Geneva, World Health Organization; 2020. https://www.who.int/biologicals/vaccines/TCV_Meeting_Report_Workshop_25_26Nov2019_FINAL.pdf?ua=1
Evaluation of Candidate International Standards for Meningococcal Capsular Group W and Y Polysaccharide. Prepared for the WHO Expert Committee on Biological Standardization, Geneva, 21-25 October 2019. Geneva: World Health Organization; 2019 (Document WHO/BS/2019.2374)
International collaborative study for the replacement of a International Standard for the WHO 1st International Standard for Haemophilus influenzae type b capsular polysaccharide.Prepared for theWHO Expert Committee on Biological Standardization, 13-17 October 2017. Geneva, World Health Organization; 2018 (DocumentWHO/BS/05.2018) http://apps.who.int/iris/bitstream/10665/137471/1/WHO_BS_2014.2239_eng.pdf?ua=1
Evaluation of candidate International Standards for Vi polysaccharide from Citrobacter freundii and Salmonella enterica subspecies enterica serovar Typhi. Prepared for the WHO Expert Committee on Biological Standardization, 17-20 October 2017.Geneva, World Health Organization; 2017 (Document WHO/BS/2017.2310)
http://www.who.int/biologicals/expert_committee/BS2310_Vi_PS_Report_for_WHO_Final.pdf?ua=1
Evaluation of candidate International Standards for Meningococcal Serogroups A and X polysaccharide. Prepared for theWHO Expert Committee on Biological Standardization, 12-16 October 2015. Geneva, World Health Organization; 2015. (Document WHO/BS/2015.2255 Add 1)
http://www.who.int/biologicals/expert_committee/BS2255Add_MenA_MenX_PS_Report.pdf?ua=1
Evaluation of a candidate International Standard for Meningococcal Serogroup C polysaccharide. Prepared for theWHO Expert Committee on Biological Standardization, 17-21 October 2011. Geneva, World Health Organization; 2011. (Document WHO/BS/2011.2169)
World Health Organization. http://www.who.int/iris/handle/10665/70860