STR (Short Tandem Repeat) Analysis

STR loci are defined regions of DNA containing multiple copies of short sequences of bases (typically 3 to 7 bases) which are repeated a number of times; the number of repeats varying among individuals in the population.

Note: Due to the banding pattern the samples are distinct (different number of repeats at each loci)

The following data represents DNA fingerprints of STR (short tandem repeat) loci for our reference materials. This is to ensure that reference materials are from a species specific source and are distinct.
STR loci are among the most informative polymorphic markers in the genome.

The STR analysis procedure used at the CJD resource Centre involves the simultaneous amplification of eight STR loci and the amelogenin gene in a multiplex PCR reaction (Promega PowerPlex® 1.2 system, Promega Corporation). This allows for discrimination of fewer than 1 in 10⁸ individuals (Lins, A.M., et al. JOFS. 43: 1168, 1998). The amplicons are separated on an ABI 310 Prism Capillary Genetic Analyser and data collected using Genescanning® software (Applied Biosystems).

Each peak in the resulting electropherogram represents an allele, is compared to an allelic ladder profile.

Comparative alignment of traces (and manually read repeat numbers) have shown the samples are distinct and therefore different.


Please Note: The CJD Resource Centre does not endorse, warranty the use of, or represent the products or processes used to obtain the following data for any purpose. The data below is provided on a 'as is' basis and we advise recepients of samples who demand a specific DNA profile to recharacterise the samples.

Please note: Amelogenin is not a STR loci and the data below just indicates size of the band.

Sample: NHBZ00005 - Clear

This data is no longer available on this site.
However the samples are distinct and free from cross contamination.