The following alignment image image was generated after direct sequencing from PCR products using template genomic DNA extracted from the following homogenates;
Reference Code
| Ref in image
| Chr$
|
---|
NHSY0/0009
| NHSY0/0009
| nvCJD(M/M)
|
NHSY0/0009a
| NHSY0/0009
| nvCJD(M/M)
|
NHSZ0/0012
| NHSZ0/0012
| -ve Ctrl(V/V)
|
NHSZ0/0012a
| NHSZ0/0012
| -ve Ctrl(V/V)
|
Since these sequences represent data from a number of experiments (both + & -ve strand sequencing) the term composite is used to describe them.
Table of examples of SCF trace data (SCF data captured as image) at codon 129.
Genotype at codon 129
|
|
MM
|
|
MV
|
|
VV
| |