The following alignment image image was generated after direct sequencing from PCR products using template genomic DNA extracted from the following homogenates;
Reference Code
| Ref in image
| Chr$
|
NHSY0/0009 |
NHSY0/0009 |
nvCJD(M/M) |
NHSY0/0009a |
NHSY0/0009 |
nvCJD(M/M) |
NHSZ0/0012 |
NHSZ0/0012 |
-ve Ctrl(V/V) |
NHSZ0/0012a |
NHSZ0/0012 |
-ve Ctrl(V/V) |
Since these sequences represent data from a number of experiments (both + & -ve strand sequencing) the term composite is used to describe them.
Table of examples of SCF trace data (SCF data captured as image) at codon 129.
Genotype at codon 129 |
|
MM |
|
MV |
|
VV |
|