The following alignment image image was generated after direct sequencing from PCR products using template genomic DNA extracted from the following buffy coat fractions;
Reference Code
| Ref in image
| Chr$
|
---|
NSFP0/0201 | Sheep1a, Sheep1b | Scrapie ARQ,VRQ |
NSFP0/0205 | Sheep2a Sheep2b | Scrapie ARQ,VRQ |
NSFZ0/0209 | Sheep3a, Sheep3b | Ctrl ARR,ARQ |
The image includes markers to localise the position of codon136, codon154 and codon171. A change in the relative last but one base is the position of the polymorphism.
![](/image.ashx?assetid=eb106e14-f8d0-4772-bd32-a77eaa3fd25a)