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  • HPLC, Mass Spec and Sequence
 

Peptide Master Datasheet

The following data was supplied with the peptides by the manufacturer

 
Reference Code
Sequence
Data
HPLC
MassSpec
NPOA0/0100H-KKRPKPGGWNTGGSRYPGQG-OH100
100
100
NPOA0/0101H-TGGSRYPGQGSPGGNRYPPQ-OH101
101
101
NPOA0/0102H-SPGGNRYPPQGGGGWGQPHG-OH102
102
102
NPOA0/0103H-GGGGWGQPHGGGWGQGGGTH-OH103
103
103
NPOA0/0104H-GGWGQGGGTHSQWNKPSKPK-OH104
104
104
NPOA0/0105H-SQWNKPSKPKTNMKHMAGAA-OH105
105
105
NPOA0/0106H-TNMKHMAGAAAAGAVVGGLG-OH106
106
106
NPOA0/0107H-AAGAVVGGLGGYMLGSAMSR-OH107
107
107
NPOA0/0108H-GYMLGSAMSRPIIHFGSDYE-OH108
108
108
NPOA0/0109H-PIIHFGSDYEDRYYRENMHR-OH109
109
109
NPOA0/0110H-DRYYRENMHRYPNQVYYRPM-OH110
110
110
NPOA0/0111H-YPNQVYYRPMDEYSNQNNFV-OH111
111
111
NPOA0/0112H-DEYSNQNNFVHDCVNITIKQ-OH112
112
112
NPOA0/0113H-HDCVNITIKQHTVTTTTKGE-OH113
113
113
NPOA0/0114H-HTVTTTTKGENFTETDVKMM-OH114
114
114
NPOA0/0115H-NFTETDVKMMERVVEQMCIT-OH115
115
115
NPOA0/0116H-ERVVEQMCITQYERESQAYY-OH116
116
116
NPOA0/0117H-TQYERESQAYYQRGSS-OH117
117
117
NPOA0/0118H-AAGAVVGGLGGYVLGSAMSR-OH118
118
118


Please note NPOA0/0103 is a composite sequence based around the octapeptide repeat region.

NP0A0/0118 and NPOA0/0107 are essentially the same except for amino acid residue 13. NP0A0/0118 has a Valine residue, and NPOA0/0107 a methionine.

NPOA0/0120 is a peptide panel of 19 vials comprising of one of each peptide vial
 
All High Performance Liquid Chromatography (HPLC) has the following conditions 
Variable
Conditions
Column
Vydac 218TP; 4.6mm x 250mm
Solvent A:
0.1% Trifluroacetic acid in water
Solvent B:
0.1% Trifluoroacetic acid in acetonitrile
Gradient
(a) 5% - 95% B over 20min

(b) 10% - 30% B over 20min
Flow rate:
(a) 1.5cm3.min-1 (b) 1.5cm3.min-1
Chart Speed
5mm.min-1
Temperature
20oC
Detection
230nm
Absorbance
1.28A

 

Ref.Code
NPOA0:		Sequence
HPLC
MassSpec
0100
H-KKRPKPGGWNTGGSRYPGQG-OH
affiniti1:9.48,13.03
affiniti2:5.90,20.47		Calculated MW 2128.3Da
Observed molecular ion (M+H)+ 2128.3Da
no other significant ions
0101
H-TGGSRYPGQGSPGGNRYPPQ-OH
affiniti1:6.3,6.72,7.73,9.62
affiniti2:5.55,6.39,6.98		Calculated MW 2033.1Da
Observed molecular ion (M+H)+ 2033.0Da
no other significant ions
0102
H-SPGGNRYPPQGGGGWGQPHG-OH
affiniti1:5.39,5.72,6.12
affiniti2:14.42,17.85		Calculated MW 1963.0Da
Observed molecular ion (M+H)+ 1963.0Da
no other significant ions
0103
H-GGGGWGQPHGGGWGQGGGTH-OH
affiniti1:9.58,9.86,10.82
affiniti2:2.72,6.32,7.15,8.28		Calculated MW 1803.8Da
Observed molecular ion (M+H)+ 1804.3Da
no other significant ions
0104
H-GGWGQGGGTHSQWNKPSKPK-OH
affiniti1:6.31,6.89,7.05
affiniti2:9.29		Calculated MW 2094.2Da
Observed molecular ion (M+H)+ 2094.4Da
no other significant ions
0105
H-SQWNKPSKPKTNMKHMAGAA-OH
affiniti1:6.82,7.05
affiniti2:6.26,7.48,8.12,8.91		Calculated MW 2212.5Da
Observed molecular ion (M+H)+ 2213.2Da
no other significant ions
0106
H-TNMKHMAGAAAAGAVVGGLG-OH
affiniti1:9.22,9.9310.33,
11.58,12.33,12.83
affiniti2:8.08,8.70		alculated MW 1784.0Da
Observed molecular ion (M+H)+ 1784.5Da
Other significant ions (M+Na)+ 1806.6Da, (M+K)+ 1829.5Da
0107
H-AAGAVVGGLGGYMLGSAMSR-OH

affiniti1:4.47,5.10,7.47,8.76,

9.25,9.76
,11.8,12,12,12.75
,13.08,13.24,14.16
affiniti2:8.35,10.89

Calculated MW 1825.0Da
Observed molecular ion (M+H)+ 1825.2Da
no other significant ions
0108
H-GYMLGSAMSRPIIHFGSDYE-OH

affiniti1:7.92,8.11,8.50,8.80,

9,9.49.75
affiniti2:10.09,11.06,11.57

Calculated MW 2231.4Da
Observed molecular ion (M+H)+ 2231.4Da
no other significant ions
0109
H-PIIHFGSDYEDRYYRENMHR-OH
affiniti1:8.62
affiniti2:11.06,11.4,11.79		Calculated MW 2598.7Da
Observed molecular ion (M+H)+ 2599.2Da
no other significant ions
0110
H-DRYYRENMHRYPNQVYYRPM-OH
affiniti1:8.76,9.76
affiniti2:2.87,4.90,7.31,7.78,
8.96,16.14,18.02		Calculated MW 2752.0Da
Observed molecular ion (M+H)+ 2752.8Da
no other significant ions
0111
H-YPNQVYYRPMDEYSNQNNFV-OH
affiniti1:8.27,8.59,12.13
affiniti2:12.05		Calculated MW 2541.6Da
Observed molecular ion (M+H)+ 2542.2Da
no other significant ions
0112
H-DEYSNQNNFVHDCVNITIKQ-OH
affiniti1:4.6,6.74,7.73,8.3
,8.72,8.94,13.97,21.12
affiniti2:6.60,9.17		Calculated MW 2381.5Da
Observed molecular ion (M+H)+ 2381.9Da
Other significant ions (M+Na)+ 2303.9Da
0113
H-HDCVNITIKQHTVTTTTKGE-OH

affiniti1:4.57,10.49,10.88,

11.32,18.82
affiniti2:7.39,8.41,8.70,

8.96,19.30

Calculated MW 2226.4Da
Observed molecular ion (M+H)+ 2249.8Da
no other significant ions
0114
H-HTVTTTTKGENFTETDVKMM-OH

affiniti1:6.78,7.4,7.52,8.10,

8.96,14
affiniti2:6.39,6.97,8.298.83

Calculated MW 2271.5Da
Observed molecular ion (M+H)+ 2272.4Da
Other significant ions (M+Na)+ 2294.5Da
0115
H-NFTETDVKMMERVVEQMCIT-OH
affiniti1:6.39,8.35,10.10
affiniti2:7.36,7.77		Calculated MW 2404.7Da
Observed molecular ion (M+H)+ 2405.1Da
no other significant ions
0116
H-ERVVEQMCITQYERESQAYY-OH

affiniti1:7.96,8.26,8.80,18.37,

18.57
affiniti2:5.80,7.45,8.08,8.34

Calculated MW 2525.7Da
Observed molecular ion (M+H)+ 2525.7Da
no other significant ions
0117
H-TQYERESQAYYQRGSS-OH
affiniti1:6.46
affiniti2:2.93,6.87,7.32		Calculated MW 1952.9Da
Observed molecular ion (M+H)+ 1953.1Da
no other significant ions
0118
H-AAGAVVGGLGGYVLGSAMSR-OH

affiniti1:7.83,8.50,8.97,

18.38,18.56
affiniti2:8.21,9.49,10.94

Calculated MW 1793.0Da
Observed molecular ion (M+H)+ 1793.1Da
no other significant ions

Please note NPOA0/0103 is a composite sequence based around the octapeptide repeat region.

NP0A0/0118 and NPOA0/0107 are essentially the same except for amino acid residue 13. NP0A0/0118 has a Valine residue, and NPOA0/0107 a methionine.

NPOA0/0120 is a peptide panel of 19 vials comprising of one of each peptide vial

 

All High Performance Liquid Chromatography (HPLC) has the following conditions

 

Variable
Conditions
Column
Vydac 218TP; 4.6mm x 250mm
Solvent A:
0.1% Trifluroacetic acid in water
Solvent B:
0.1% Trifluoroacetic acid in acetonitrile
Gradient
(a) 5% - 95% B over 20min

(b) 10% - 30% B over 20min
Flow rate:
(a) 1.5cm3.min-1 (b) 1.5cm3.min-1
Chart Speed
5mm.min-1
Temperature
20oC
Detection
230nm
Absorbance
1.28A


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