Coomassie Gel

Introduction
Samples were run on a gel and coomassie brilliant blue stained to demonstrate protein purity.

Protocol

Protein preparation
Samples were prepared as serial dilutions in PBS (phosphate buffered saline).An equal volume of 2X denaturation gel loading buffer was added and samples were heated to 95°C for 5 mins. A total of 20µl of sample was loaded onto a 16% Tris-glycine gel that was run for 60 mins at 100 volts, 120mA.

Coomassie gel and Visualisation
Gels were stained with coomassie brilliant blue, washed with destain and visualised by UVP image analysis

Figure: Coomassie gel