NIBSC - Product details

ID	1049
Title	DU174
Characteristics	Infect 10x106 normal donor PBMCs, previously stimulated for three days with PHA. Exchange 50% of the medium every 3-4 days. Once each week, add 5 x 106 PHA-stimulated normal donor PBMCs. Freeze supernatant 10 days into the co-culture. Propagate in RPMI 16
Source	Prof Lynn Morris
Reference	Swanstrom R.AIDS Res Hum Retroviruses. 2003 Feb;19(2) 133-44