We develop flow cytometry reference materials to standardise clinical diagnostic assays and harmonise flow cytometric analysis between laboratories. Existing flow cytometry reference materials cover the following areas:
Flow cytometry cross-matching (FCXM) is routinely performed to match donors and recipients of transplants and in transfusion medicine. It determines the level of reactive antibodies in patients’ serum directed against donor HLA antigens – usually by using donor lymphocytes as targets. The presence of antibodies indicates that the recipient has been sensitised to donor antigens, increasing risk of rejection.
Transfusion-related acute lung injury (TRALI) can cause of illness and death following blood transfusion. It results from an immune reaction between the recipient and the transfused blood. Antibodies in donor plasma act against the recipient’s white cells and/or endothelial cells leading to leukocytes being trapped in the small blood vessels of the lung and damage to the pulmonary endothelium. This results in fluid and inflammatory cells leaking into the lungs. The antibodies are normally directed against the recipient’s human leucocyte antigen (HLA) class I, HLA class II or human neutrophil antigens (HNA).
Our flow cytometry cross-matching (FCXM) CE-marked reference materials are developed as positive and negative control standards for human leucocyte antigen (HLA) miss-match screening to minimise the risk of Transfusion Related Acute Lung Injury (TRALI). A list of our available reference materials can be found at the top right of this page. View an overview of both available and in development FCXM reference materials.
We produce lyophilised antibodies and cell standards for diagnostic assays for the enumeration of specific cell populations.
µg with 3.6 FITC molecules/IgG
15/270CD4 WHO Reference Reagent
We can also provide the mouse anti-human CD4 monoclonal antibody conjugated to FITC, for use in laboratories performing diagnosis of HIV/AIDS and monitoring the effectiveness of HIV antiretroviral therapy (ART).We have developed a stable reference material containing stabilised granulocyte colony-stimulating factor (G-CSF) mobilized apheresis cells that can be used as a quality control product for flow cytometry CD34+ stem cell counting. The CD34+ cell standard (available from USP catalogue) supports a US Pharmacopeia sub <1000> General Test Chapter for CD34+ cell enumeration by flow cytometry.
CD4 T cell counting using the FITC CD4 Positive Control Cells (SS-319/20) and TrucountTM beads
Dr Sandra Diebold, Section Leader Dr Anna Nowocin, Head of Flow Cytometry StandardisationDr Sandrine Vessillier, Head of ImmunotoxicologyDr Deepa Rajagopal
11/210: FITC SS-319/20: FITC CD4 Positive Control Cells10/142: Negative plasma control for HLA cross matching08/148: HLA class II positive plasma control07/214: Minimum potency positive plasma control for FCXM15/270: CD4 WHO Reference Reagent