The following alignment image image was generated after direct
sequencing from PCR products using template genomic DNA extracted
from the following buffy coat fractions;
Reference Code
|
Ref in image
|
Chr$
|
NSFP0/0201
|
Sheep1a, Sheep1b
|
Scrapie ARQ,VRQ
|
NSFP0/0205
|
Sheep2a Sheep2b
|
Scrapie ARQ,VRQ
|
NSFZ0/0209
|
Sheep3a, Sheep3b
|
Ctrl ARR,ARQ
|
The image includes markers to localise the position of codon136,
codon154 and codon171. A change in the relative last but one base
is the position of the polymorphism.