The following alignment image image was generated after direct sequencing from PCR products using template genomic DNA extracted from the following homogenates;
Reference Code
| Ref in image
| Chr$
|
NHSY0/0009 |
NHSY0/0009 |
nvCJD(M/M) |
NHSY0/0009a |
NHSY0/0009 |
nvCJD(M/M) |
NHSZ0/0012 |
NHSZ0/0012 |
-ve Ctrl(V/V) |
NHSZ0/0012a |
NHSZ0/0012 |
-ve Ctrl(V/V) |
Since these sequences represent data from a number of experiments (both + & -ve strand sequencing) the term composite is used to describe them.
![](/image.ashx?assetid=99c4dbd6-c525-43ee-96e5-91d98e9d11ae)
Table of examples of SCF trace data (SCF data captured as image) at codon 129.
Genotype at codon 129 |
|
MM |
![](http://www.nibsc.ac.uk/spotlight/cjd/dataimages/MMexample.jpg) |
MV |
![](http://www.nibsc.ac.uk/spotlight/cjd/dataimages/MVexample.jpg) |
VV |
![](http://www.nibsc.ac.uk/spotlight/cjd/dataimages/VVexample.jpg) |