NIBSC - Product details

ID	6014
Title	HIV-1 Rev (Wild Type)
Characteristics	The rev cDNA from pCV-1 (NIH Cat #303) was amplified using PCR and cloned into the expression vector pOTS Nco1 (SmithKline Beecham). This plasmid was transformed into E. coli N5151 and thermally induced. The crude Rev was re-suspended in Buffer A (50 mM T
Source	Mr. M. J. Orsini, Dr. D. Rekosh, and Dr. M. L. Hammarskjöld.
Reference	AIDS Res Hum Retroviruses 11:945-953, 1995.