For each material, hover the "+" cursor over the dilution curve at the mutation percentage required to see the dilution to be performed.
For example, to prepare a further standard of consensus mutation percentage 50% for material 16/252 (KRAS p.Gly12Ala), hover the "+" cursor over 50% on the curve to see the dilution required i.e. 1.30 means that a 1 in 1.30 dilution (1 part of material 16/252 plus 0.30 parts of wild- type KRAS codons 12 and 13, material 16/266) will yield a further standard of consensus mutation percentage 50% KRAS p.Gly12Ala, e.g. 8μl of material 16/252 plus 2.4μl of material 16/266.
* The KRAS consensus mutation % is shown at 5 decimal places to ensure the accuracy of the dilution curves. Users are likely to be working with a maximum 1 or 2 decimal places so rounding may be required.
Do not export the graphs for your calculations. Calculations are only valid when made on this webpage.